Lymphat Res Biol: Development of a Porcine Lymph Drainage Model to Study Particle Migration

Spenciner DB, Barber FA, Zani BG, Melidone R, Muench TR. “Development of a Porcine Lymph Drainage Model to Study Particle Migration.”

Summary: Intra-articular wear particulate migration from the knee joint has been studied in various animal models as well as postmortem in patients who received total knee joint replacement. However, there still exists a need for a simple, yet analogous animal model for tracking the migration of wear debris from the knee joint, especially through the draining lymph nodes.

To fill this need, a proof-of-concept porcine model was developed for particle migration from the knee joint into the surrounding lymphatic system. Vitreous carbon particles were deposited both intra-articularly and extracapsularly in a bilateral manner to the hind limbs in pigs (n = 6). The regional/draining lymph nodes were qualitatively assessed weekly by a veterinarian by manual palpation to detect any enlargement or change in consistency when compared to the initial assessment before the surgical procedure. At 6 weeks, the draining lymph nodes were harvested and processed for histology. Microscopic evaluation showed carbon particle migration from the knee into 100% of the iliac lymph nodes, 50% of the inguinal lymph nodes, and 0% of the popliteal lymph nodes.

Overall, this study established a needed animal model for evaluating carbon particle migration to the draining lymph nodes from the knee joint.

Lymphat Res Biol. 2019 Aug;17(4):418-423. doi: 10.1089/lrb.2017.0088.

August 17, 2019