"Intracochlear administration methods across species." Molly Kalker, Benny Muraj, Marion Souchal, Ellie Khadir, Gaëlle Naert, Aurore Marie, Donald Hodges, Rami Tzafriri, Sebastien Diot
Inner ear delivery offers several advantages for gene therapy, including reduced viral titers, non-systemic administration, and improved transduction efficiency. Delivery can be performed via the round window (RW), posterior semi-circular canal (PSCC), or intra cochleostomy (IC), each providing access to specific inner ear compartments.
PSCC injections target both endolymphatic & perilymphatic spaces, while RW and IC approaches primarily deliver into the perilymph in adult animals. These techniques are technically challenging due to the small size, delicate
structure, and deep anatomical location of the inner ear, requiring high surgical precision and species-specific anatomical knowledge.
This poster highlights the cross-species application of these techniques, showcasing successful delivery via PSCC and RW injection in mice, intracochlear (cochleostomy) infusion in guinea pigs, and RW injection in swine.
Poster presentation at ASGCT 28th Annual Meeting, May 13-17, New Orleans